Genomics
Human Reviewed
Single-cell RNA sequencing reveals novel transcriptional programs in regulatory T cells during acute inflammatory response
Chen, S., Patel, R., Müller, K., Zhang, W. et al.
We present a comprehensive single-cell transcriptomic analysis of over 48,000 regulatory T cells isolated from peripheral blood of patients with acute inflammatory conditions. Our analysis reveals 6 novel cell states not previously described...
Neuroscience
AI Reviewed
CRISPR-Cas9 mediated knockout of PTEN in hippocampal neurons enhances spatial memory consolidation in aged mice
Rodriguez, M., Kim, J., Okonkwo, F.
Age-related cognitive decline remains a significant challenge in neuroscience. Here we demonstrate that selective PTEN deletion in CA1 hippocampal neurons using AAV-delivered CRISPR-Cas9 results in a 34% improvement in Morris water maze performance...
Microbiology
Awaiting Review
Antibiotic resistance gene transfer via outer membrane vesicles in clinical Klebsiella pneumoniae isolates
Hassan, A., Johansson, L., Bose, S., Williams, T.
Horizontal gene transfer mediated by outer membrane vesicles (OMVs) represents an underappreciated mechanism of antibiotic resistance dissemination. Using whole-genome sequencing and electron microscopy, we characterize OMV-mediated carbapenem resistance transfer in clinical isolates...
Biochemistry
Human Reviewed
Structural basis of allosteric regulation in human pyruvate kinase M2 by fructose-1,6-bisphosphate
Liu, Y., Andersen, P., Gupta, N.
Pyruvate kinase M2 (PKM2) plays a central role in the Warburg effect observed in cancer cells. We present cryo-EM structures of PKM2 in its active tetrameric and inactive dimeric states at 2.4 Å resolution, revealing a novel allosteric mechanism...
Cell Biology
AI Reviewed
Mitochondrial dynamics in brown adipocyte differentiation: a role for Drp1-mediated fission in thermogenic capacity
Yamamoto, K., Sorensen, E., Ibrahim, R., Costa, M.
Mitochondrial morphology is dynamically regulated during adipogenesis, yet the functional significance of fission and fusion in thermogenic adipocytes remains unclear. Here we show that Drp1-mediated fission is required for uncoupling protein 1 expression and full thermogenic capacity...
Immunology
Human Reviewed
Tissue-resident memory T cells in the intestinal epithelium maintain long-term protection against enteric pathogens independent of circulating immunity
Nakamura, T., Bell, A., Ferreira, P., Singh, R.
The relative contributions of circulating and tissue-resident memory T cells to intestinal immune protection remain poorly understood. Using parabiosis and intravascular staining, we demonstrate that Trm cells in the intestinal epithelium are both necessary and sufficient for rapid pathogen clearance...